Debabrata Chowdhury
Indian Institute of Chemical Technology, India
Title: Insights into the role of a newly identified plausible biomarker of cardiac hypertrophy
Biography
Biography: Debabrata Chowdhury
Abstract
Cardiac hypertrophy leading to heart failure is one of the major causes of morbidity and mortality in the world. It is characterized by a chronic physiological increase in cardiac muscle mass resulting from systolic or diastolic wall stress. It can be induced in rats by administration of isoproterenol (ISO), a catecholamine. Therefore, in our recent study we used a proteomics based approach (two dimensional electrophoresis followed by MALDI-TOF MS/MS analysis) a) to analyze and contrast the proteome of the healthy and ISO induced hypertrophy in male Sprague Dawley rats and b) to identify potential biomarkers for diagnosis of cardiac hypertrophy. The above study resulted in the identification of different proteins of major functional groups such as cholesterol and protein metabolism, muscle contraction and development, transport, TCA cycle, ATP-biosynthesis, chaperone, signal transduction, DNA synthesis and ubiquitinisation. We were also able to identify and study differentially expressed proteins in the diseased sample which revealed that prohibitin (PHB) differentially expressed protein which is mainly associated with cardiac muscle growth and development. The present aim of the study is therefore to investigate the function of this protein at molecular level to understand and elucidate its direct or indirect involvement in cardiac disease progression or cardio protection. Nevertheless, the findings from the present study we observed that this protein is mainly involved in ROS signaling pathway and cardio protective effect in in-vitro and could be useful to predict the therapeutic response to establish novel therapies for the prophylaxis of cardiac hypertrophy and subsequent heart failure in cardiac patients.