Translational Medicine

Biography

Biography: Manoj Kumar

Abstract

Background: Alternation of hematopoietic has been observed in severe trauma and hemorrhagic shock. Hemorrhagic Shock (HS) induced expression of Hypoxia Inducible Factor (HIF)-α, β results elevated Erythropoietin (EPO) level. Endogenously elevated EPO unable to reactivate the hematopoietic failure. The purpose of the study was to evaluate the serum EPO levels and expression of the erythropoietin receptor (EPO-R) on Bone Marrow (BM) that can prediction of delay recovery of hematopoietic dysfunction. Objective: This study to evaluate the serum levels EPO and expression of bone marrow EPO-R in patients with trauma hemorrhagic shock. Methods: We conducted a prospective study with 100 individuals of whom 30 (%) were healthy controls and 70 (%) were T/HS. Day 1, we have collected peripheral blood sample for measurement of EPO levels by sandwich ELISA and those patients who have survived day 3, collected bone marrow sample for analysis of EPO-R expression on BM by flow cytometry. Clinical and laboratory data were prospectively collected. Results: Significantly decreased the expression of EPO-R (<0.05) on bone marrow in the T/HS patients when compared with control group. Serum levels of EPO did not significant in T/HS patients. Conclusions: Our studies suggest, expression of EPO-R on BM can be used predictor of delay recovery of hematopoietic dysfunction. Further investigate in this area must determine the expression of EPO-R mRNA on BM by RT-PCR and more studies to be needed exogenously effect of EPO on hematopoietic dysfunction in patients with T/HS.